Zeiss Axiovert 135M Manual de usuario descargar pdf (Pagina 3)

System Anatomy

The Axiovert 135 is a manually operated microscope with automated digital image capture. The

objectives, emission filter cubes, DIC prisms, and focus are all controlled manually. The software is only

for image capture and processing, it does not run any aspect of the microscope.

Before changing the magnification, lower the objectives by focusing the objective turret all

the way down, so as not to disturb the sample or bash the objectives into the stage. There are three

objectives available on this microscope: PlanNeoFluar 20x NA 0.5 dry, PlanNeoFluar 40x 1.30 oil, and

PlanApoChromat 63x 1.40 oil. NA refers to Numerical Aperture, the resolving ability of the lens; bigger

NA = higher resolution.

Brightfield and Fluorescence modes

There are six positions for fluorescence filter

cubes in the slider below/behind the objectives (Fig. 2, “filter”).

The filter position is changed by pushing the slider back and

forth. The cube in the light path can be determined using the

chart on the front of this manual. Since this is a manual

microscope, the excitation/emission cubes must be changed by

hand with the slider. Fluorescence uses excitation filters to

excite your probe(s) with a narrow band of wavelengths, a

dichroic mirror to reflect the excitation onto your sample while

allowing the longer fluorescence to pass trough, and a barrier

(AKA emission) filter to block stray excitation light and limit

the output color. All of the filter cubes on this microscope use

Fig. 2 The ND and filter sliders

filter

Fig. 1

The

Zeiss Axiovert135 inverted light microscope

1 2 3 4 5 6

Sin comentarios

Zeiss Axiovert 135M Manual de usuario Pagina 3