Zeiss LSM 510 META Manual de usuario

Zeiss LSM 510 inverted confocal manual Power Up 1. Turn on the mercury lamp (if required) 2. PC/system 3. Components 4. Logon to ASADMIN Open the So

Z-sectioning To collect a series of images at different points along the z-axis the settings can be inputted in two ways: Z Sectioning or Mark Fir

8. Start will begin scanning of the z stack. Settings last entered in the Channels and Mode panels will be applied to this z stack collection. 9. The

Time series The Time Series is a versatile software function that is useful in a variety of situations - from collecting single confocal images or ent

Ending your session Remove your sample from the stage and clean any oil objectives you used. Please let us know of any problems, the Report Problem fo

3-D stack display options Animate Some users will find it helpful to assess their time or z-stack data by animating it. 1. Select Anim from the Sele

toolbar. Here, define new start and end points of the series using the Start Slice and End Slice sliders/data fields, or enter a value in the Eve

monochrome (grayscale) if Raw data single or Raw data series image type is chosen. 6. Click Save. When a z- or time stack file is exported, each stac

Program Start: - Select the configuration you wish the software to load by default every time you log on. - Un-select "don't show logo"

When you've located something you'd like to confocal, click the LSM button on the software panel. Acquiring a confocal image Turn On the L

Turn on only the laser(s) you need to excite the fluorophores in your sample. To turn on the Argon laser, click standby to warm it up. After the

1. Single- or multi-track? Single Track mode is for single channel imaging or simultaneous excitation of multiple channels; in Multi Track mode the l

Adjustments under the channels tab . . . 1. Adjust % laser transmission(s) in the Excitation panel at the bottom of the window. Doing so sets the %

4. Click the Fast XY button and while the laser is scanning, use the XY Stage Controller and adjust the fine focus on the microscope body. Clicking

8. Reset the Palette to "No Palette".

Adjustments under the mode tab . . . Most of these will be fine as the default values. Averaging is the most important thing to adjust. 512*512 pixe

The zoom and scan field rotation can be set in the Zoom, Rotation, & Offset panel (Offset here refers to fine XY movement of the scan area). You m