Zeiss LSM 510 META Manual de usuario descargar pdf (Pagina 5)

Adjustments under the channels tab . . .

1. Adjust % laser transmission(s) in the Excitation panel at the bottom of the window. Doing so sets the

% laser power let through by the AOTF.

2. Set the pinhole diameter with the Pinhole slider scale. The smaller the pinhole, the thinner the

optical slice will be and the less light will be collected. A pinhole set to 1 Airy unit (click the 1 button) will

have optimal resolution. However, you will probably need to adjust this according to your sample's

fluorescence, using larger Airy values for dim samples. For multi-fluorophore imaging, it is usually

advisable to set the pinholes for each channel to produce equivalently thick optical slices.

3. Press "Find" to acquire a preliminary image using automatic setting of gain. An image display

window will open and the output of the scan will be displayed. If you are performing multi-channel

imaging, select Split XY button in the "Image" window's Display toolbar.

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Zeiss LSM 510 META Manual de usuario Pagina 5