Time series
The Time Series is a versatile software function that is useful in a variety of situations - from collecting
single confocal images or entire z stacks over time to more sophisticated applications such as FRAP. If
desired, a delay between the beginning of one scan process to the beginning of the next can be set. It
is important to note that Time Series works at only one location on the slide. To apply the Time Series
function to multiple stage locations, you will have to use the Multitime macro.
The following instructions are for applying a basic Time Series. It is assumed that the user has
optimized and set all imaging parameters beforehand (pinhole size, detector gain/offset, scan time,
averaging, and if desired, z stack). Refer to previous sections for more details on adjusting these
settings.
1. Select the Acquire>Time Series submenu.
2. In the Start Series panel, choose between a Manual start or a timed start via Time. Most users want
a manual start. If a timed start is desired, enter a start time in the Time (h:m:s) field. The time series
will start when the set time is reached according to the computer's clock.
3. In the End Series panel, enter the number of time points desired in the Number field if a Manual end
is selected. For a timed end point, depress Time and enter the end time in the Time (h:m:s) field.
In determining an experiment's length and the number of time points needed it is important to consider
the scan time and averaging settings (see the Speed section of the Mode panel in "Scan
Control"). Both parameters influence how long it takes the laser to clear the scan field and thereby
define the minimum time between time points.
4. The next panel you encounter will be either Time Interval or Time Delay, depending on the settings
of your Time Series register (see Options--> Settings). Time Delay is defined as the end of one scan
process to the beginning of the next, while Time Interval is the time between the beginning of one scan
process to the beginning of the next. Either panel, Time Interval or Time Delay, permits time series
scan parameters to be activated or changed from experiment to experiment.
You may load & Apply previously saved time series settings using the pulldown menu in either of these
panels. Or you may choose to manually enter scan parameters into the Time and Unit fields. If you
foresee performing other experiments with identical time scan settings, you should Store your manually
entered settings to the dropdown list for easy reference in the future.
5. Click Start T in the right-hand side of the "Time Series" window to start your time series.
6. The collected time series is temporarily stored in the computer's memory. To permanently store the
series, use Save or Save As in the image display window to save it to a database.
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