Zeiss LSM 510 META Manual de usuario descargar pdf (Pagina 11)

8. Start will begin scanning of the z stack. Settings last entered in the Channels and Mode panels will

be applied to this z stack collection.

9. The collected z stack is temporarily stored in the computer's memory. To permanently store the

stack, use Save or Save As in the image display window to save it to a database.

Z Sectioning Method

1. This method assumes the user has prior knowledge of the specimen's thickness and/or is willing to

guesstimate thickness. Select Z Stack at the top in the "Scan Control" window - doing this will activate

the Z Settings panel.

2. Click on the Z Sectioning tab and select Keep Interval. This will keep the z slice size constant, even

if you later decide to change the Num Slices or the upper and lower limits in your stack.

3.Type in the desired interval for your z stack in the Interval (um) field of the Z Sectioning tab. If

necessary, look up the optimal z interval thickness by clicking Z Slice at the top of the Z Settings

box. This will open the "Optimal Slice" window. Assuming you have already adjusted your pinholes as

described in "Acquire Menu: Collect An XY Section", your optimal z interval thickness for the objective

currently in use will be displayed in this window. Clicking Optimal Interval in the lower left corner of the

window will set the z interval of your z stack to the recommended thickness.

4. The Num Slices will be calculated automatically based on the chosen interval input.

5. Designate your current focal position (if you're in focus, you're probably somewhere near the middle

of the specimen) in the Current Slice field.

6. Clicking Start will begin scanning of the z stack. Settings last entered in the Channels and Mode

panels will be used to collect this z stack collection.

7. The collected z stack is temporarily stored in the computer's memory. To permanently store the

stack, use Save or Save As in the image display window to save it to a database.

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Zeiss LSM 510 META Manual de usuario Pagina 11