Zeiss LSM 510 META Manual de usuario descargar pdf (Pagina 3)

Turn on only the laser(s) you need to excite the fluorophores in your sample.

To turn on the Argon laser, click standby to warm it up. After the initial warm-up period, the on button

will appear allowing the laser to be fully activated. Set the output intensity to an appropriate level - 40%

is a good starting point.

To turn on any of the other lasers, simply click the appropriate "On" buttons - no warm-up is required

for ignition.

We try to minimize the number of times a laser is turned on or off - if somebody will use the laser within

2 hours leave it on (Argon laser in standby). Check the reservation schedule to see the laser usage

scheduled before and after your session. Turn off any lasers you don't need that may have been left on

for you by the previous user, as long as anyone scheduled after you won't use them within 2 hours of

your imaging session.

Choose a configuration

A filter configuration for the particular fluorophores you are using can be loaded via the "Configuration

Control" window. Open it by selecting the Acquire>Config submenu.

Laser

Lines

(nm)

Colour of

fluorophores

Examples of

fluorophores

405

Diode

405 Deep blue DAPI

Argon

458 477

488

514

Green (Cyan

and

Yellow)

GFP, Alexa 488,

FITC,

CY2

561

Diode

561 Red

Alexa 568, TRITC,

CY3

594

gas

594 Red

Alexa 594, texas

red, mCherry

633

gas

633 Far-red Alexa 633, CY5

1 2 3 4 5 6 7 8 ... 16 17

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Zeiss LSM 510 META Manual de usuario Pagina 3

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