Zeiss LSM 510 META Manual de usuario descargar pdf (Pagina 6)

4. Click the Fast XY button and while the laser is scanning, use the XY Stage Controller and adjust

the fine focus on the microscope body. Clicking "Stop" in the Scan Control window will turn off the

Fast XY continuous scanning (minimize bleaching when you can).

The preliminary image, whether too dim or too bright, usually needs to be optimized. The PMTs need

to be adjusted according to the fluorescence intensity of the sample.

5. Select each channel separately by clicking on the corresponding coloured button.

6. The range indicator display is helpful for setting the gain and offset. Select Palette in the image

window's Select toolbar. From the Color Palette List, select "Range Indicator". In this palette, pixels

with intensity of 0 are blue and pixels with intensity of 255 (for 8 bit images) are displayed red.

7. Start Fast XY and set the Detector Gain until no more than a few % of your stained areas has red

pixels. Move the Amplifier Offset until the background is a mixture of blue and black pixels. (Always

leave the amplifier gain as 1). Make these adjustments for each channel.

1 2 3 4 5 6 7 8 9 10 11 ... 16 17

Sin comentarios

Zeiss LSM 510 META Manual de usuario Pagina 6