Ending your session
Remove your sample from the stage and clean any oil objectives you used. Please let us know of any
problems, the Report Problem form at
http://microscopy.duke.edu/problem.html is quick to use.
Check the
on-line reservation schedule to see if/when the next reservation is scheduled:
If someone else is scheduled to use the microscope within 2 hours...
Put the Argon laser into standby and turn off any lasers that won't be used by the next person,
leaving on hte ones indicated on the booking calendar
Exit the software
Select the "Log Off" command from the Start menu of the desktop and leave the system on.
Sign the logbook
If you are the last scheduled session of the day or for more than 2 hours...
Turn off all the lasers in the Acquire>laser box and exit the software
Select "Shut down computer" command in the Start menu of the desktop. The computer and
monitors will power down automatically.
Turn off the Components switch
Turn of the PC/system switch
Turn of the arc lamp
Sign the logbook
Image Processing & Analysis
Scalebars & Measurements
A scale bar, measurements (e.g. angle, area, line) and/or text can easily be drawn onto an image
that has already been collected using the Overlay option in the image display window. You are
encouraged to explore the drawing buttons in the Overlay toolbar - creating a scalebar on your
image is described here.
1. Click Overlay in the Select toolbar of an open image window.
2. Select the desired scalebar thickness from the line width option buttons appearing half way down
the Overlay image window submenu.
3. Click on the desired color of your scale bar from the color selection at the bottom of the Overlay
submenu.
4. Click 1um in the Overlay submenu, then click in the vicinity of the image where you wish the
scalebar to be drawn.
5. Drag the mouse over some distance on the image until the resulting scalebar registers your
desired value. Release the mouse button to draw this scalebar overlay on the image.
6. Use Save or Save As in the image window to save it to a database.
(120 paginas)
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