
KF 2
ICS
Carl Zeiss
B 40-028 e 10/97 3-3
3
OPERATION
3.1 Choice of objectives
The objectives are the centerpiece of the microscope. The following is an example of how objectives can
be labeled:
CP-"Achromat" 10 /0.25 /-
where
10 = objective magnification
0.25 = numerical aperture
= infinite tube length
- = usable for covered and uncovered specimens
or
0.17 = only for covered specimens with a cover slip thickness of 0.17 mm
The objective magnification multiplied with the eyepiece magnification (minimum 10x) results in the
visual overall magnification: e.g. 10 10 = 100 .
The numerical aperture 1000, e.g. 0.25 1000 = 250, is the highest useful magnification, i.e. no
further details are resolved above that limit.
The " "sign means that these objectives cannot be used with microscopes the objectives of
which are labeled with "160" for the mechanical tube length.
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