Zeiss Axiovert 200 Manual de usuario

Busca en linea o descarga Manual de usuario para Microscopios Zeiss Axiovert 200. QSG-WF1 - Workspace Manual de usuario

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27 Jan 2015,<qsg-wf1> p. 1/8
Q
UICK
S
TART
G
UIDE
:
W
IDEFIELD
1
Z
EISS
A
XIOVERT
200
/
V
OLOCITY
(SAFB
409)
Startup procedure ................................................................................................................ 2
Observing through the eyepiece .......................................................................................... 2
Brightfield: ................................................................................................................................... 3
Fluorescence: ............................................................................................................................. 3
Adjusting brightfield Köhler illumination ............................................................................... 3
Acquiring the image ............................................................................................................. 4
Multidimensional setup ........................................................................................................ 5
Multiple channels ........................................................................................................................ 5
Z stacks ...................................................................................................................................... 5
Time series ................................................................................................................................. 6
Multi-position ............................................................................................................................... 6
Manual positioning .................................................................................................................................... 6
Automated positioning .............................................................................................................................. 6
Stitching ...................................................................................................................................... 6
Start Capture ............................................................................................................................... 7
Shutdown procedure ............................................................................................................ 7
Other controls ...................................................................................................................... 8
Auto Exposure ............................................................................................................................ 8
Auto Contrast .............................................................................................................................. 8
Keypad controls: ......................................................................................................................... 8
File formats .......................................................................................................................... 7
Facility for Imaging
by Light Microscopy
Observing Life As It Happens
Imperial College London
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Indice de contenidos

Pagina 1 - Imperial College London

27 Jan 2015,<qsg-wf1> p. 1/8 QUICKSTART GUIDE: WIDEFIELD 1 ZEISS AXIOVERT 200 / VOLOCITY (SAFB 409) Startup procedure ...

Pagina 2 - Startup procedure

27 Jan 2015,<qsg-wf1> p. 2/8 Startup procedure Turn on (in this order): • if required: heater unit and heater, 4 hours before live imaging to

Pagina 3 - Fluorescence:

27 Jan 2015,<qsg-wf1> p. 3/8 The shutters can be controlled either in the software or on the microscope Brightfield: - Select channel 1 in Vo

Pagina 4 - Acquiring the image

27 Jan 2015,<qsg-wf1> p. 4/8 Acquiring the image • On the microscope stand - change the light path to BASEPORT • In Volocity - select channel

Pagina 5 - Multidimensional setup

27 Jan 2015,<qsg-wf1> p. 5/8 Multidimensional setup Multiple channels • open "Acquisition Setup" dialogue • tick "Change cha

Pagina 6 - Automated positioning

27 Jan 2015,<qsg-wf1> p. 6/8 Time series • in "Acquisition Setup" select the “Time” tab • set intervals • set duration Multi-p

Pagina 7 - File formats

27 Jan 2015,<qsg-wf1> p. 7/8 Start Capture To start(/stop) recording - click on the red button icon on the video toolbar you start your record

Pagina 8 - Other controls

27 Jan 2015,<qsg-wf1> p. 8/8 Other controls Auto Exposure • sets max pixels to 80% (not 100%) (can also be applied to a selected area) Auto C

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