Zeiss Axiovert 200 Manual de usuario descargar pdf (Pagina 7)

27 Jan 2015,<qsg-wf1> p. 7/8

Start Capture

To start(/stop) recording - click on the red button icon on

the video toolbar you start your recording as defined in

"Acquisition Setup"

File formats

• Images are automatically saved to the library – there is no “Save” option

• Individual images can be renamed by clicking on the image name

• The original Volocity libraries are stored as "mvd2" files together with a folder containing the image data

in "aisf" files (these cannot be opened). These libraries can be opened either by using Volocity (offline-

licenced server – this is charged by the hour) or with FIJI or ICY (free).

• Right click on image to export

• Exported TIFF: loses all enhancements but keeps original ("raw") data

- All stacks/ channels are stored in 1 multipage TIFF with the original bit-depth (8 or 12 bit)

• Exported TIFF for publication

- Screen version of image, including colours, merge, enhancements, on-screen image resolution,

it is always 8-bit



loss of original ("raw") data!!!

Shutdown procedure

• check if anyone is booked after you within 2 hours

If nobody is booked within two hours:

• remove specimen

• clean oil objective lenses with lens tissue

• Important: close incubation chamber completely ( protects from dust)

• update time in Sharepoint

• secure your data (e.g. copy them to the server)

• shut down hardware in reverse order from startup (making sure Fluorescent lamp is switched off even if

you have not used it)

If someone’s booked within two hours:

• update usage in Sharepoint

• remove your samples

• clean objective lenses with fresh lens tissue and close incubation chamber

• clear up the desk

• save files onto the server or on a mobile hard drive

• log off

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Zeiss Axiovert 200 Manual de usuario Pagina 7