Zeiss LSM 510 META Manual de usuario Pagina 2
- Pagina / 56
- Tabla de contenidos
- MARCADORES
- Confocal microscopy 1
- Turning on the lasers 7
- (Main menu: Acquire) 10
- Contents 13
- Multi Track Configuration 17
- Cy5-Cy3-FITC Multi Track 18
- 633 nm, using 18
- 1) Select Scan 19
- 3) Select the Frame Size 19
- = 1 Airy unit 23
- Pinhole size 23
- Image Acquisition 24
- Optical Zooming 26
- 1) Select Palette 27
- 2) Select Range Indicator 27
- Red = Saturation (maximum) 27
- Blue = Zero (minimum) 27
- Photobleaching is linear! 29
- Adjusting the Laser Intensity 30
- Adjusting Gain and Offset 31
- Line Averaging 34
- Frame Averaging 35
- Collecting an Averaged Image 36
- Confocal Z Sectioning 40
- Z - Series using Z Sectioning 42
- Z Sectioning – Setting Range 43
- Viewing a Z - Series 44
- 1) Select Gallery 45
- 2) Select Data for 45
- Use Subset to 45
- Multiple Regions of Interest 49
- Time Series 50
- 1) If required, use 52
- Saving Data - Using Database 54
- Saving Data – Using Export 55
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