Zeiss Axiolab A Manual de instrucciones Pagina 50
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Carl Zeiss Axiolab A
3-8 B 40-015 e 06/99
(3) Transmitted-Light Polarization - Determination of the n
γ
γγ
γ
'
Vibration Direction and
Determination of the Path Difference
Fig. 3-6 Determine the n
γ
γγ
γ
'
vibration direction using the example of an artificial fiber
Application
The position of the two directions with the relatively highest (n
γ
'
) and the relatively lowest (n
α
'
) refractive
index, or the absolutely highest (n
γ
) and the absolutely lowest (n
α
) refractive index, in relation to
morphological directions of crystal surfaces, crystal needles or fibers, is an important criterion for
recognition. It is also used, for example, for the diagnosis of biocrystals (gout, pseudogout).
Settings
• Set the microscope as usual for transmitted-
light brightfield examinations.
• Turn stage until the first stop position, unlock
stop and turn the stage until the object features
maximum darkness.
• Activate stage stop mechanism and turn stage
around 45° until the next stop so that the
longitudinal axis of the fiber is oriented in NE-
SW direction.
• Allocate a path difference of approx. 150 nm to
this color on the MICHEL LEVY color chart.
− When the λ-compensator (3-5/2) has been
pushed in, the color of the fiber changes to
yellow-orange (path difference approx. 400
nm).
− When the stage is turned around 90°, the
fiber appears greenish blue (path difference
approx. 700 nm).
150 700 -400
λ
γ
λ
γ
γ
γ
γ
Fig. 3-7 Schematic diagram of the color
chart in addition and subtraction
position
- Axiolab A 1
- Reflected-Light 1
- Microscope 1
- Carl Zeiss Axiolab A 10
- X B 40-015 e 06/99 10
- DESCRIPTION 11
- 1-6 06/99 B 40-015 e 16
- 1-8 06/99 B 40-015 e 18
- 1-10 06/99 B 40-015 e 20
- Axiolab A Carl Zeiss 23
- B 40-015 e 06/99 1-13 23
- B 40-015 e 06/99 1-15 25
- 1-16 06/99 B 40-015 e 26
- B 40-015 e 06/99 1-17 27
- 1-24 06/99 B 40-015 e 34
- START-UP 35
- 2-2 B 40-015 e 06/99 36
- OPERATION 43
- 3-2 B 40-015 e 06/99 44
- 150 700 -400 50
- Fig. 3-11 Stage selection 57
- CONTAX 167 MT 61
- 4-2 B 40-015 e 06/99 68
- Fig. 4-1 Changing the fuses 70
- B 40-015 e 06/99 A-1 73
- A-2 B 40-015 e 06/99 74
- A-4 B 40-015 e 06/99 76
- T slow-blow fuse type 76
- TV television 76
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