Zeiss Axiovert 135M Especificaciones descargar pdf (Pagina 45)

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL

B 40Ć012 e 08/99 2Ć11

Height adjustment of the condenser 0.55

The height of the condenser 0.55 can be

adjusted in order to obtain homogeneous

illumination of the object field and optimum

intensities in all the focusing positions of the

objective.

When the fixation screw has been loosened, the

lower part of the condenser can be moved in the

direction of the upper part and clamped in any

position.

In phase and VAREL contrast, the phase rings

and full diaphragms remain flush after the

adjustment.

• Condenser in low position if the object plane

is 0...2.5 mm above the stage surface.

• If the object plane is more than 2.5 mm above the stage surface, the lower part of the condenser

must be moved upwards and clöamped in the object field when optimum illumination intensity

has been achieved.

2.4.2 Brightfield illumination

• Preparations as described in sections 2.4. and

2.4.1.

• Set Ph/H/Ph slider (2Ć14ā/ā4) to center position.

• Place the object on the stage and focus at low

magnification, e.g. using the CP Achromat

10x objective, via the focusing drive.

• Close the aperture diaphragm via the lever

(2Ć14ā/ā6) until optimum contrast has been

achieved.

NOTE The aperture diaphragm cannot

be used to control the image

brightness (loss of image quality)!

• If required, change the lamp voltage via control (2Ć14ā/ā5) or insert attenuation filter (2Ć14ā/ā1) in

the filter slider (2Ć14ā/ā2) and push it in the beam path.

Figure 2Ć13   Condenser 0.55

Figure 2Ć14Various modes of brightfield illumination

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Zeiss Axiovert 135M Especificaciones Pagina 45