Zeiss Axiovert 135M Especificaciones descargar pdf (Pagina 48)

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL

08/99 B 40Ć012 e

2Ć14

2.4.4 VAREL contrast

VAREL contrast provides a reliefĆlike image of specimens and can be used as an alternative to phase

contrast. VAREL contrast can also be used on curved surfaces, e.g. 96 microtiter plates, for which no

contrast can be achieved with phase contrast (no flush ring positioning!).

• Preparations as described in section 2.4 and

2.4.1.

• Open the aperture diaphragm (2Ć18

ā/ā1)

completely.

• Insert the specimen with the suitable holding

frame in the holding clips (2Ć18

ā/ā4) of the

specimen guide.

• From the right, push the Phā/āHā/āVar slide

(2Ć18ā/ā3) into the slider mount on the 0.4 or

0.55 condenser up to the VAREL position.

• Swivel in the required VAREL objective.

• Use the adjusting screw (2Ć18ā/ā5) to move the

left or right VAREL diaphragm opening (after

selecting the engaging position) until

optimum VAREL contrast is achieved (reliefĆ

like impression).

NOTE Microtiter plates:

Select the opposite VAREL ring of

the diaphragm for illumination at

the edge of the cavity; in the

middle of the cavity, the left or

right VAREL ring can be used.



Figure 2Ć19 VAREL contrast with microtiter plates

Specimen field with relevant pupil image of the VAREL diaphragm

(diaphragm appears rotated by 180°)

Figure 2Ć18 VAREL contrast observation

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Zeiss Axiovert 135M Especificaciones Pagina 48