Zeiss LSM 510 META Manual de usuario descargar pdf (Pagina 12)

LSM 510 Meta Training Notes

Single Track

z Select Channel Mode for normal

scanning and Single Track. Follow the

excitation/emission pathway in the

image to define your configuration.

z Press Excitation to select laser line

and % transmission.

z Press and select an Excitation

dichroic; this selection depends on the

laser lines being used to excite the

sample.

z Select 1

and 2

dichroics; selection is

based upon wavelengths you want to

capture. Dichroics reflect short

wavelengths and pass long wavelengths.

z Activate detectors; ChS, Ch2 or Ch3

and select psuedocolor for display.

ChD is for transmitted light.

Excitation dichroic

Emission Filters

Detectors

and 2

dichroics

Transmitted light

detector

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Zeiss LSM 510 META Manual de usuario Pagina 12