Zeiss LSM 510 META Manual de usuario descargar pdf (Pagina 19)

LSM 510 Meta Training Notes

Z-Stack Settings

z Adjust settings at optimal plane of focus.

z Select Mark First/Last tab

z Use the fast scan setting to focus down

through image until image fades; press

Mark First.

z Use the fast scan setting to focus up

through image until image fades; press

Mark Last.

z Select Z-Slice and then press Optimal

Interval to ensure correct sampling. The

Z-slice is calculated to be ½ of the Z-

resolution (Optical Slice on Channels tab.).

z Press Start to begin collecting the stack.

The entire stack of images will be saved as

one file.

Note scan

buttons are

different

under Z-

settings

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Zeiss LSM 510 META Manual de usuario Pagina 19