Zeiss Axiovert 200 Guía de usuario descargar pdf (Pagina 12)

Figure 13

Camera settings in AxioVision software

In the image window there is a "French flag" button in the tool bar at the bottom (Fig. 12).

This will show under/overexposed areas in blue/red pseudocolor. Pressing the button again

removes the pseudocolor.

3.2.2.2 White balance

White balance adjustment is not needed in fluorescence imaging because there is only one

color at a time. However, inappropriate white balance settings may lead to poor image

quality. It is recommended to choose 3200K for fluorescence imaging (Fig. 13).

Correct white balance settings are essential when taking images with transmitted light. The

color of halogen lamp depends on voltage. The color turns from red to blue when voltage is

increased. Human eye compensates for the color variation but the camera should be

adjusted properly. There are three options (Fig. 13):

Interactive... = you have to click on a spot in live image that will then represent white color

3200K = white balance will be adjusted according to color temperature 3200K

Automatic = software estimates suitable white balance for the image

3.2.2.3 Image resolution

There is a list of available camera resolutions for acquisition in BASIC ADJUSTMENT (Fig. 13).

Note that there are three options available for color images: BINNED, STANDARD and

SCANNED. BINNED and STANDARD are faster to take but SCANNED offers better image

quality. When using BINNED resolutions, pixels close to each other are combined. This

results in higher sensitivity (and shorter exposure times) but lower resolution.

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Zeiss Axiovert 200 Guía de usuario Pagina 12