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1. General
1.1 Instrument
This user guide covers the use of the Zeiss AxioVert 200 inverted epifluorescence
microscope. For more information on each MIU instrument, visit:
http://www.miu.helsinki.fi/instruments/index.htm
An inverted microscope is usually used for observing living cells on culture dishes and bottles
that do not physically fit under an upright microscope. In an inverted microscope, the
condenser is above the specimen plane and the transmitted light source (halogen bulb) is
above the condenser. In an inverted epifluorescence microscope, there is also a light source
(usually a mercury bulb) for reflected light that enables detection of fluorescence signal from
the specimen.
1.2 Reservations
Instructions for Scheduler online reservation system can be found on the MIU web page:
http://www.miu.helsinki.fi/reservations.htm. You can make your reservations two weeks in
advance. The minimum time block you can reserve is 15 min. If you need to cancel your
reservation, it must be done before your reservation starts. Unused and uncancelled
reservations will also be charged.
If you want to extend your session and there are no reservations after you, you can use the
"extend" option in Scheduler (click first on your reservation). If your reserved time is already
up, you cannot extend your present reservation any more.
Make sure you are familiar with the use of Scheduler and MIU user policy and fees
(http://www.miu.helsinki.fi/userinfo.htm and http://www.miu.helsinki.fi/fees.htm).
1.3 Billing
The billing is based ONLY on the reservations made in Scheduler - the logbook markings do
not count. The fee for the one hour training session is 21 € for internal users (July 2008).
Check the current rate on the MIU Fees web page. Any time after the initial training, you can
ask MIU staff to give you additional customized training (charged the same way as the initial
training).
1.4 Unauthorized use
MIU periodically checks the logins of the computers. If your logon/off times exceed your
reserved time in Scheduler, MIU will consider that as unauthorized use of the instrument
and will ask for an explanation. For unauthorized use of the instrument, MIU may issue a
warning. After three warnings, MIU may revoke the user's license. Also, misuse/neglect of
the instrument may lead either to a warning or cancellation of the user license, and the
repair costs may be charged.
(56 paginas)
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