Zeiss Axiovert 200 Guía de usuario descargar pdf (Pagina 19)

The whole image is too bright:

Is the transmitted light (halogen bulb) off (p. 5)?

Cannot see the whole field of view:

Is the fluorescence filter correctly in place? Is the shutter for fluorescence light completely

out of the light path (p. 5)?

6.1.2 Transmitted light microscopy

You cannot see anything:

Is the halogen light on (p. 5)? Is the intensity of the light adjusted properly (p. 5)?

The image is too bright and colored:

Check that the shutter for fluorescence light is closed (Fig. 3, p. 5).

Cannot see the whole field of view:

Check that the filters above the condenser are not partially on the light path. Is the

fluorescence light shutter completely closed?

Transmitted light image is too red / too blue:

Is DAPI filter selected? Is the fluorescence light shutter closed? Illumination is uneven =>

adjust Koehler illumination (p. 7). Check white balance settings (chapter 3.2.2).

Phase contrast image is not good:

Are you using a phase contrast objective (Table 1, p. 6)? Does the condenser position match

the objective (pages 6 and 8)? Is your specimen thin enough for phase contrast imaging?

DIC image is not good:

Are you using an objective suitable for DIC imaging (p. 4)? Does the condenser position

match the objective (p. 6 and 8)? Is the DIC slider in position and correctly adjusted (Fig. 9, p.

9)? Is your specimen in a suitable container for DIC viewing (no plastic)?

6.2 Camera

It’s not possible to acquire an image:

Check that the camera is on (chapter 3.1). If the camera was turned on after starting the

software you should exit and restart the software.

Camera image is not in the focus as the image viewed through the oculars:

Adjust the oculars according to your vision (Fig. 2, p. 5).

1 2 ... 14 15 16 17 18 19 20

Sin comentarios

Zeiss Axiovert 200 Guía de usuario Pagina 19