Zeiss Axiovert 200 Guía de usuario descargar pdf (Pagina 7)

2.4 Transmitted (bright-field) light microscopy

Transmitted light microscopy is used for specimens that are stained, or have natural pigment

so they are able to absorb light. Koehler illumination ensures that illumination is

homogeneous and devoid of disturbing scattered light. Thus, adjustment of Koehler

illumination is essential every time transmitted light microscopy is done. Switching

objectives requires readjustments

For transmitted light imaging, make sure that the halogen light pathway is open and that

there is no fluorescence filter in the light pathway (select position 6 in the filter wheel that

has no filter).

Bright-field imaging is usually used for histologically stained specimen but sometimes

unstained specimen can also be observed. All objectives are suitable for brightfield imaging.

For the 10x and 20x objectives, the condenser should be in the position DIC .3-.4. For the 40x

and 63x objectives, select DIC.5-1.4.

Change the condenser position by manually turning the black ring in the condenser (Fig. 5).

You can see the selected condenser position in the window in the front of the condenser

(Fig. 5, red circle).

2.4.1 Koehler illumination

Koehler illumination is needed for achieving optimal illumination. Proceed as follows:

Figure 5

The position of the condenser

Figure 6

Adjusters for field diaphragm (1)

Condenser elevation (2)

Condenser centering (3)

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Zeiss Axiovert 200 Guía de usuario Pagina 7