4
1.5 Users’ mailing list
All registered users are automatically added to the users’ mailing list. Please read those e-
mails to know what is going on!
1.6 User guide
This user guide will be continuously updated by the MIU personnel. Check that you have the
latest version of the user guide: go to http://www.miu.helsinki.fi/instruments/ and compare
the date of the latest update with the date on the first page of your printed user guide. This
is the only place for update announcements; no e-mails about updates will be sent.
The user guide can also be accessed in the computer through the Internet Explorer browser:
http://www.miu.helsinki.fi/instruments/.
1.7 Acknowledging MIU
Whenever you are using instruments and/or services maintained/provided by MIU, you are
expected to acknowledge MIU in your publications and inform MIU about the papers.
1.8 Image processing and analysis
MIU has two Imaging Workstations that have software for further image processing and
analysis, such as deconvolution, cell counting, and more. Visit the MIU web site or contact
MIU for more information. Use of Imaging Workstations is free of charge, and MIU staff
gives training and help with the software.
In case the commercial and free software do not satisfy your image analysis needs, MIU
provides customized image analysis tools.
2. Microscope use
Put the cell culture bottle or plate on the microscope stage above the objective. If it does
not physically fit there, move the condenser up using the knob shown in Fig. 6.
If you are viewing cells or tissues on an objective slide, place it upside down on the objective
slide holder, and attach the holder onto the microscope stage by pushing it into the holder
frame. Turn the two adjustment wheels connected to the stage on the right-hand side so
that the part you want to view is over the objective.
2.1 Settings for the light path
Turn on the microscope using the green switch on the right side of the microscope. Adjust
the knob on the left side of the microscope between VIS for the oculars and FP for the
camera (Fig. 1, arrow 1).
Rotate the wheel so that a circle is displayed to direct the path of light to the oculars and
front-mounted AxioCam camera (Fig. 1, arrow 2).
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