Zeiss Axiovert 200 Guía de usuario descargar pdf (Pagina 8)

- Select a 10x objective and DIC .3-.4 condenser position by rotating manually the

condenser wheel

- Focus on the specimen

- Close luminous field diaphragm until its edges can

be seen through oculars (Fig. 6, a wheel in front of

the top part of the condenser).

- Focus the image of diaphragm edges by moving

the condenser up or down (Fig. 6, arrow 2).

- Use centering screws to move the diaphragm

image to the center of the field of view (Fig. 6,

arrows 3).

- Open the luminous field diaphragm until its edges

are outside the field of view (Fig. 6, arrow 1).

- Remove one ocular by pulling it out and close the

aperture diaphragm until it is about 80% of the

diameter of the objective pupil. The aperture

diaphragm is adjusted by moving left/right the black level above the condenser

position window in the front of the condenser.

When adjusting Koehler illumination for other objectives, it is sufficient just to adjust

the luminous field diaphragm and the aperture diaphragm without taking out the

ocular.

2.4.2 Phase-contrast imaging

Check that is objective is suitable for phase-contrast imaging. Rotate the condenser so that

the matching condenser position (Ph1 or Ph2) is shown in the front (Fig. 5).

2.4.3 DIC imaging

Turn the polarizer into to the light path (Fig. 9). The polarizer is a round filter above the

condenser.

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Zeiss Axiovert 200 Guía de usuario Pagina 8