Zeiss LSM 510 META Manual de usuario descargar pdf (Pagina 44)

Carl Zeiss - Training Application and Support Center

Zeiss LSM 510 META - Guided Tour

44

Scanning a Z-Series using Mark First/Last

1) Select Z Stack

2) Start scanning using Fast XY or XY cont

3) Keep your eye on the image and move the

focus to the beginning of the Z-Series, then

select Mark First

4) Move the focus back in the opposite direction

to the end of the Z-Series, then select Mark Last

5) X:Y:Z = 1:1:1 sets the Z-interval so that the

voxel has identical dimensions in X, Y, and Z.

6) Start will initiate the acquisition of the Z-

Stack. The acquisition can be stopped at any

time.

NOTE

Focusing can be achieved manually (preferred),

or using Stage in the LSM menu if there is a

motorized scanning table.

Focus

Increment

1 2 ... 39 40 41 42 43 44 45 46 47 48 49 ... 65 66

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