Zeiss LSM 510 META Manual de usuario Pagina 54
- Pagina / 66
- Tabla de contenidos
- MARCADORES
- Confocal microscopy 1
- Confocal Principle 2
- LSM 510 scan head 3
- Axioplan 2 imaging 3
- Axiovert 100M 4
- LSM 510 META 5
- Axiovert 200M 6
- Turning on the lasers 12
- Contents 15
- Fluorescence is observed by 17
- Choosing the configuration 20
- Multi Track Configuration 23
- Cy5-Cy3-FITC Multi Track 24
- Adjusting the scan speed 27
- Image Acquisition 30
- Pixel sizeMagnification 31
- Optical Zooming 32
- 1) Select Palette 33
- 2) Select Range Indicator 33
- Red = Saturation (maximum) 33
- Blue = Zero (minimum) 33
- Saturation at the maximum 34
- Gain set correctly 34
- Amplifier Gain increases the 34
- will need to be decreased 34
- Photobleaching is linear! 35
- Adjusting the Laser Intensity 36
- Adjusting Gain and Offset 37
- Line Averaging 40
- Frame Averaging 41
- Collecting an Averaged Image 42
- Confocal Z Sectioning 46
- Z - Series using Z Sectioning 48
- Z Sectioning – Setting Range 49
- Viewing a Z - Series 50
- 1) Select Gallery 51
- 2) Select Data for 51
- Use Subset to 51
- Multiple Regions of Interest 55
- Time Series 56
- Tiled Image 60
- Saving Data - Using Database 62
- Saving Data – Using Export 63
- Shut Down Procedure 64
- Please consult the manual 65
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