Zeiss LSM 510 Manual de usuario descargar pdf (Pagina 10)

7. Optimizing the settings

Due to the sequential nature of ‘Multi-track’

acquisition optimizing the settings in this mode is

difficult. It is easier to set the imaging parameters

for each Track individually. You can do this by

switching off each track with the checkbox

along side it.

Turn off all but one channel now.

7.1. Getting the display ready

This current type of display does not allow fine tuning of the imaging parameters and

so needs to be adjusted.

Optimizing the confocal settings is best done with the image pseudo-colored to

highlight saturated and zero value pixels.

To do this, first click Palette on the image window tool bar and the Color Palette

window will open.

Click Range Indicator and close the window.

Now you will have an image where red represents the saturated pixels (i.e. =255) and

the blue represents the black pixels (i.e. zero).

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Zeiss LSM 510 Manual de usuario Pagina 10