Zeiss LSM 510 Manual de usuario descargar pdf (Pagina 23)

10.4. FRAP

1. Click on Edit Bleach toolbar button to open up Bleach Control dialog.

2. Set Bleach parameters in Bleach Control dialog

Check Bleach after Number of Scans

Set “Scan Number” to for pre-bleach imaging

Set “Iterations” value – this may require empirical

determination. It needs to be enough to allow complete

bleaching but few enough to allow quick return to imaging.

Try 10 as a first attempt.

In “Excitation of Bleach Track” set bleaching laser to

100%

3. Click Define

Region button to select

bleach area

4. Define area to bleach then select it by

ticking the checkbox along side the ROI.

Close dialog.

5. Open Time Series Control dialog by clicking the

TimeSeries button in the main toolbar.

5. Set FRAP time-course in Time Series Control Dialog

Start Series = Manual.

Stop Series = select Time then enter the duration of the

experiment.

Enter Cycle Delay (time between frames).

7. Click “StartB” to start experiment.

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Zeiss LSM 510 Manual de usuario Pagina 23