Zeiss LSM 510 Manual de usuario descargar pdf (Pagina 15)

7.3. Acquiring your final image

Once you are satisfied that each channels detector is set optimally to the range of the

image, you can create your final image. Ensure that all Tracks are checked when

acquiring the final image.

Go to the Scan control window and click the Mode button.

Change the Frame size by clicking on the Optimal button (see Appendix 1 for an

explanation of this).

Noise can be reduced by averaging a

number of frames and slowing the scan

speed.

In the first instance, try the following values:

Mode: line

Method: mean

Number: 2

Scan speed: 6

Change the image Palette back to No Palette.

Click Palette

on the image window tool bar and the Color Palette window will open.

Click No Palette and close the window.

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Zeiss LSM 510 Manual de usuario Pagina 15