Zeiss LSM 510 Manual de usuario descargar pdf (Pagina 13)

You need to set the detector range to match the dimmest and brightest signal

from the specimen. Setting the detector incorrectly results in the loss of

information from the specimen.

3. Set Ampl. Offset – setting the ‘min’ signal

This should be set next, but may need re-setting later. While acquiring with the Fast

scan button, adjust the Amp. Offset so that only a few of the pixels in the background

of the image are blue. If you have a lot of blue colour in the background, move the slider

to the right.

4. Set Detector Gain and Excitation

power – setting the ‘max’ signal

The ‘max’ signal is set by adjusting the

detector gain and laser

transmission simultaneously. You

need to empirically work out the best

laser power settings – low laser power

causes less bleaching but requires the

detector gain to be set high; which

introduces noise.

Whist acquiring with the Fast scan button, adjust increase the Detector Gain so that

you get a bright image, but not too many red (saturated) pixels and not too much noise.

You may need to adjust the Ampl. Offset if you increase the Detector Gain a lot.

Around 600 is a good start. This should be set in conjunction with the excitation

intensity.

5. Set Ampl. Gain

Leave as 1 unless you have a very dim signal and nothing else works. This will amplify

noise as well as signal.

1 2 ... 8 9 10 11 12 13 14 15 16 17 18 ... 25 26

Sin comentarios

Zeiss LSM 510 Manual de usuario Pagina 13