
6
4. Find the specimen
(Axioplan 2)
The light path select,
focus knob
and stage
are manual controls. The rest of the microscope
is controlled by the software.
To set up the Axiovert microscope to locate your
specimen first move the light path selector to
VIS.
Move the correct objective in to place.
Do not use air-objectives after an
immersion objective without wiping the
immersion liquid (water or oil) from the
specimen and objective.
Brightfield, halogen light can be switched on and off HAL button
on the body of the microscope.
The intensity of brightfield light is most easily adjusted with the
Intensity control knob on the body of the microscope
Arc lamp light can be shuttered on and off by either the FL button
on the body of the microscope on the right hand-side.
The fluorescence filter block carousel can be switched back
and forward using the two buttons on the body of the microscope,
underneath the left hand focus
knob.
Once you have found your specimen, pull the light path
selector out to the LSM position. The microscope will
automatically change the filter-position and shutter the light
sources. If you fail to do this you will receive an error when you
try to acquire a confocal image.
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