Zeiss LSM 510 Manual de usuario descargar pdf (Pagina 22)

DIC

DF Ph

10.3. Transmitted light image

Whilst the laser is scanning the field of view a

certain amount of the excitation laser light passes

through the specimen. This light can be detected

and its intensity in each part of the field of view

corresponds to the transmitted light optical

properties of the specimen. In this way a

“brightfield” transmitted image can be

reconstructed. It is not a “confocal” image in that

the light does not come from a single plane.

DIC – differential interference contrast

BF – bright field

DF – dark field

For this, the microscope transmitted path needs to

be set correctly. Open the Microscope Control

panel (Toolbar Acquire then Micro)

The Field Stop iris needs to be fully opened –

100%.

The Filter at 100%

The transmitted light to be at 0%.

Click o the Condensor button to reveal the

condensor options

The Condensor Filter needs to be set to the

appropriate type of brightfield i.e. BF, DIC,

DF or Ph.

NOTE: The software keeps changing the

condensor settings back to “BF”. Keep

checking that it is set correctly.

Then, whilst in fast scan mode, adjust the transmitted light channel’s (ChD) detector gain

and offset so that the background of the image is mid-grey and the brightest part of the

image is near saturation (i.e. shows red with the Range Indicator palette).

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Zeiss LSM 510 Manual de usuario Pagina 22