Zeiss LSM 510 META Manual de usuario Pagina 5

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Zeiss LSM 510 Meta Confocal Microscope Instructions
Copyright 2013 University Instrumentation Center UNH
W123 Parsons Hall 23 Academic Way, Durham, NH 03824
Phone: 603-862-2790 Fax: 603-862-4104
www.unh.edu/research/uic
Halogen
Lamp
Focu
s
fin
e
coars
e
Kno
b
d. Put slide on stage in the depression at front of stage insert.
All of our objective lenses are designed to be used with
#1.5 coverslips.
e. Push the slide forward, under the two metal clips (to no particular distance; just so the slide is held
by the clips).
f. Using joystick, position sample under objective lens.
g. If you lowered stage by tapping Load position, return stage to work
position by tapping
If you ever wish to establish a
new vertical work position for the
stage (e.g. because your slides are
substantially thicker or thinner
than those of an earlier user), tap
Load position, adjust stage to new
desired work position with focus
knobs, then tap Set Work
position.
15. Viewing sample through the eyepieces.
a. Select Vis, either by pushing Vis button on left side of scope
OR
by clicking Vis button on monitor.
b. To examine your sample by transmitted light (TL), turn on
halogen lamp by rotating TL knob
clockwise until a comfortable light level is seen
through eyepieces. With TL button,
TL can be turned off and on (and it will return to the last intensity
used).
c. Locate and focus on area of interest using joystick and focus knobs.
d. If desired, increase magnification by tapping button on touchscreen
for a higher-power objective lens (as in 14b). If you select one of the
immersion objective lenses, lens will swing into place and stage will
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