Zeiss LSM 510 META Manual de usuario Pagina 7

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Zeiss LSM 510 Meta Confocal Microscope Instructions
Copyright 2013 University Instrumentation Center UNH
W123 Parsons Hall 23 Academic Way, Durham, NH 03824
Phone: 603-862-2790 Fax: 603-862-4104
www.unh.edu/research/uic
then click Find again so that two fluorescences are displayed.
Click the last channel button, then click Find yet again
so that all three fluorescences are displayed.
Click Find anytime you wish software to
attempt to set brightness/contrast for you (e.g. after
changing Scan Speed in Scan Control window).
c. To make adjustments to X, Y, and Z coordinates (to image the
precise area and focal plane of interest), click Fast XY button
(fastest scanning mode), then
adjust Z (focal plane imaged),
either by turning fine focus knob
on scope or by clicking up and
down arrows under Focus.
The increment of change with
each click is adjusted with
Focus step slider.
Adjust X and Y (area imaged) by
clicking up, down, left, and
right arrows under Stage XY
(with Fast XY scanning still on).
The increment of change with
each click is adjusted
with XY step slider.
The focal plane seen through
All
Channels
Combined
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