Zeiss LSM 510 META Manual de usuario descargar pdf (Pagina 9)

Zeiss LSM 510 Meta Confocal Microscope Instructions

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f. Though Find attempts to adjust settings for decent brightness/ contrast, you should fine-tune settings.

Click Palette button, then click on Range Indicator.

Collecting 8-bit (2

=256 gray levels) images, every

pixel will have a fluorescence intensity in the range of

0 (black) to 255 (white). With Range Indicator

selected, pixels with a value of 255 are shown in red

and those with a value of 0 are shown in blue, as in this

example. In general, settings are adjusted (as described

below) so that there are just a few, if any, red and blue

pixels (but at least close to having some red and blue

pixels, thus taking advantage of full dynamic range of

detectors).

 It is sometimes necessary to retain more red pixels

than is ideal so that other, less intensely fluorescent

structures remain visible. Somewhat more abundant

blue pixels in background areas are often desirable for achieving a dark

background.

g. Numbers of red and blue pixels are adjusted for each channel

separately. With Channels selected, click button of channel you wish to

adjust,

then click Cont.[inuous] button.

Red pixels are primarily adjusted using

Detector Gain slider

(reducing Detector Gain will reduce numbers of

red pixels).

Blue pixels are primarily adjusted using

Amplifier Offset slider

(reducing Amplifier Offset will increase numbers of

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Zeiss LSM 510 META Manual de usuario Pagina 9