Zeiss LSM 510 META Manual de usuario descargar pdf (Pagina 8)

Zeiss LSM 510 Meta Confocal Microscope Instructions

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eyepieces may not correspond exactly to the focal plane displayed on monitors, and the area

displayed on monitors never includes entire field of view visible through eyepieces; hence the need

to often make small adjustments to Z and XY, respectively.

d. If you only want to image a portion of the area

displayed in Image window, click Crop button,

then manipulate the displayed crop box to the size

and orientation you desire; for example

Now if you click Single, only the area within the

crop box will be scanned.

 Blue side of crop box indicates where the laser(s)

will begin scanning.

e. If the images in one or more channels appear noisier than you find

acceptable, noise can be reduced with scan averaging (each line

of image scanned more than once, then averaged). With Mode

selected, change Number from 1 to 2, 4, 8, or 16 times scan

averaging. You can check the effect of the selected scan

averaging by clicking Single.

Excessive noise resulting from low fluorescence intensity can also

be reduced by going to a slower (lower number) Scan Speed .

Graininess (pixelation) can be reduced by going to a higher Frame

Size.

16e

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Zeiss LSM 510 META Manual de usuario Pagina 8