Zeiss Axioplan 2 imaging and Axiophot 2 Guía de usuario descargar pdf (Pagina 13)

5.2.2 Oil usage

Only Zeiss F 158 immersion oil should be used with oil objectives. There are brown oil bottles on

the table between the microscopes 1 and 2. Lower the stage to “load position” for more room to

operate with oil by pressing the button A in the Figure 10. Drop a small amount of oil on the cover

glass. Bring the stage back to the previous level “work position” with the button B in the Figure 10.

Figure 10. Stage movement buttons

5.2.3 Cleaning oil objectives

After the imaging session clean up tables, stages, and especially the objectives; have the lights

turned on so that you can see properly. Use cotton swabs immersed in ethanol (take from the correct

bottle) to clean oil from objectives. After this, dry with a clean swab. Start the wiping in the middle

of the lens and move outwards in a spiral way. You can also use lens paper to first wipe up most of

the oil. If you use lens paper, do not rub it against the top lens of the objective. There are two

ethanol bottles; one is only for cleaning objectives, the other for cleaning tables, the stage etc. in

case there are oil spills.

6 Placing the sample

Place your sample on the specimen stage by manually widening and releasing the spring-operated

object holder (Figure 11, part a). To move the specimen stage in the XY-direction turn the two

adjustment wheels connected to the stage on the right-hand side (Figure 11, part c). Upper wheel

controls the movement in y-direction (back and forth), lower in x-direction (side to side). If you

need to rotate the stage to get the image in any wanted angle, open the rotation lock by unscrewing

it and then rotating the stage by hand (Figure 11, part b). Re-screw the lock after use!

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Zeiss Axioplan 2 imaging and Axiophot 2 Guía de usuario Pagina 13