Zeiss Axioplan 2 imaging and Axiophot 2 Guía de usuario descargar pdf (Pagina 16)

Figure 13. Buttons for changing the condenser position (1) and an opening to view the current

position (2).

There are predefined settings for each contrasting method and objective in software in TOOLS /

SETTINGS / WORKGROUP. When choosing a certain setting, the objective will be changed and

the condenser will be adjusted automatically.

8.2 Bright-field imaging

Bright-field imaging is usually used for histologically stained specimen, but sometimes unstained

specimen can also be observed. All objectives are suitable for bright-field imaging. To do bright-

field imaging, the condenser should be in position „I/H‟.

8.3 Phase contrast imaging

Only certain objectives (marked with Ph) are suitable for phase contrast imaging. The Ph-value

written on the objective and the condenser position value should match (Arabic numerals for phase

contrast). Change the condenser position with the button showed on Figure 13 part 1. Make sure the

Köhler illumination (Chapter 8.6) is done properly and the condenser diaphragm is open enough

(rightmost button on Figure 13). If you want to increase contrast on phase contrast images, use the

green filter under the condenser (the filter looks yellow).

8.4 DIC imaging

Currently there are no analyzers installed in either of the Axioplans, and therefore DIC imaging

cannot be done.

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Zeiss Axioplan 2 imaging and Axiophot 2 Guía de usuario Pagina 16