Zeiss Axioplan 2 imaging and Axiophot 2 Guía de usuario descargar pdf (Pagina 17)

8.5 The auxiliary lens for transmitted light

The button to control the auxiliary lens is right above the contrasting method indicator (number 2 in

Figure 13). The lens should be used when imaging with all but the 5x objective. If the auxiliary lens

is in the wrong position, it is impossible to do the Köhler illumination (Chapter 8.6).

8.6 Optimal settings for transmitted light imaging (Köhler illumination)

To achieve optimal image quality some adjustments should be made before imaging. Köhler

illumination needs to be adjusted in the beginning of every transmitted light imaging session. The

elevation and the centering of the condenser can be optimized as follows (Figure 14, the numbers in

the list refer to the arrows in the image):

Select a 10x objective

Select condenser position I/H

Focus on the specimen

Bring the condenser to the uppermost position (2)

Close luminous field diaphragm (1) until the small hole can be seen in oculars

Focus the diaphragm image by moving the condenser (2)

Use centering screws (3) to move diaphragm to the center

Open luminous field diaphragm (1) until its edges disappear from the field of view

Figure 14. Adjusters for field diaphragm (1), condenser elevation (2) and condenser centering

(3).

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Zeiss Axioplan 2 imaging and Axiophot 2 Guía de usuario Pagina 17