Zeiss Axioplan 2 imaging and Axiophot 2 Guía de usuario descargar pdf (Pagina 18)

9 Adjusting the oculars

Make sure that the oculars are correctly adjusted for your eyes. If you have normal vision, the zero

mark on the sides of the oculars should match the white dot. If you wear eyeglasses but want to use

the microscope without them, you must adjust the oculars according to your vision. First look at

your specimen with your right eye closed (through the left ocular). Slowly turn the eyepiece scale

ring until the image is optimally in focus. Repeat with the other eye. The scale on the oculars is the

diopter scale.

10 Acquiring images

10.1 Zeiss AxioVision software

The computer and the monitors may be turned off. Turn them on if image acquisition is needed.

Log on to the computer with your personal HYAD-username. Choose „Log on to ATKK‟. Start the

software by clicking the AxioVision-icon on the desktop. The microscope and camera should be

turned on before starting the software as the devices are recognized only during startup. You can

access the online help by pressing F1 in the software.

Before acquiring images, the setting for auto save folder should be changed. In the main window,

go to TOOLS / OPTIONS… / STORAGE and write “H:\” in the field. If you leave this empty,

temporary images during acquisition will be saved in your personal profile which has a limited

storage space. Large images fill up the profile quickly and error messages will pop up.

10.2 Live view

Enable real-time video by clicking the LIVE-button in software (Figure 15). It may be practical to

drag the live-window to the secondary display and maximize it there.

Figure 15. Live-button in AxioVision software.

The refresh rate of live image can be adjusted (Figure 16). Quality of live image depends on the

refresh rate. However, this setting has no influence on the actual image that will be taken. There are

three options for the refresh rate:

slow (good quality)

medium (medium quality)

fast (poor quality)

By right clicking the live image with right mouse button you can access more options, such as

zooming in and out in the image, etc.

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Zeiss Axioplan 2 imaging and Axiophot 2 Guía de usuario Pagina 18