Zeiss Axioplan 2 imaging and Axiophot 2 Guía de usuario descargar pdf (Pagina 23)

By clicking MEASURE (Figure 24) the software estimates the suitable time of exposure and the

preview live window pops up (Figure 25). Adjust the time of exposure manually if needed and

choose OK. Note that exposure adjustment works slightly different from the live window – the

image will not get darker if you shorten the exposure time, but it will be noisier. The image

intensities are multiplied to fit the monitor - hence the noise.

Figure 24. Exposure time setting

Figure 25. Settings for time of exposure.

Add more channels by clicking DUPLICATE (Figure 23). Channels can be acquired either one

channel at the time using SNAP button, or automatically acquiring all channels by clicking START

button at the bottom of the window. Channels can be enabled and disabled by clicking the channel

number with the right mouse button.

10.9 Z-stack imaging

Z-stacks can be acquired by choosing MULTIDIMENSIONAL ACQUISITION/Z. It is possible to

define either the center point of the stack or start and end points. The number of slices can be

entered directly or by specifying the slice distance. OPTIMAL DISTANCE –button calculates the

correct distance according to the Nyquist theory. This is important, if you are going process your

stack with 3D-deconvolution software. Note that the correct dye should be selected in

MULTIDIMENSIONAL ACQUISITION/C so the optimal distance could be calculated correctly.

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Zeiss Axioplan 2 imaging and Axiophot 2 Guía de usuario Pagina 23