Zeiss Axioplan 2 imaging and Axiophot 2 Guía de usuario descargar pdf (Pagina 20)

Figure 18. Workarea menu

Figure 19. Exposure settings

10.5 White balance

White balance adjustment is not needed in fluorescence imaging because the illumination color

does not change. However, inappropriate white balance settings may lead to poor image quality. It

is recommended to choose 3200K for fluorescence imaging (Figure 20). Correct white balance

settings are essential when taking images with transmitted light. The color of halogen lamp depends

on voltage. The color turns from red to blue when voltage is increased. Human eye compensates for

the color variation but the camera should be adjusted properly. There are three options (Figure 20):

Interactive... = white balance will be adjusted according a sample in the image. Click a point

that should be white i.e. a place with just glass between the light source and sensor. This

setting is recommended if the Automatic setting does not work well.

3200K = white balance will be adjusted to color temperature 3200K (Maximum power of

halogen lamp). This setting does not usually work for transmitted light imaging.

Automatic = software estimates suitable white balance according to the image. Often works

well. If not, use the Interactive selection.

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Zeiss Axioplan 2 imaging and Axiophot 2 Guía de usuario Pagina 20