Zeiss Axioplan 2 imaging and Axiophot 2 Guía de usuario descargar pdf (Pagina 24)

The AxioVision software is not able to use decimal numbers on the z slice thickness if the Windows

user language is not set to English as it cannot use “,” as a decimal separator. The language setting

is tied to the roaming user profile, so it depends on the user. To use decimal numbers in the z slice

thickness, change your language setting to English. If you need help with this, ask the MIU staff.

11 Post processing

11.1 Scalings and measurements

There are calibrated scalings for different objectives in the software. Scalings can be found in

MEASURE/SCALINGS. By choosing MEASURE/AUTOMATIC SCALING the correct scaling

will be added to the image automatically according to the objective.

Scalings can be exploited for drawing scale bars and measuring objects. A scale bar can be added

by clicking the scale bar icon. Color and style of the scale bar can be changed by clicking the scale

bar with right mouse button and choosing PROPERTIES/ATTRIBUTES.

There are various measurements available in MEASURE-menu. Data table of measurements can be

created by choosing MEASURE /CREATE DATA TABLE.

11.2 Shading correction

Irregular lighting can be corrected after acquisition with shading correction. Choose IMAGE

PROCESSING/ ADJUST/SHADING CORRECTION. There are two ways to carry out the shading

correction: taking a reference image or using auto-mode.

When using transmitted light the reference image could be an empty area of the slide. For

fluorescence light a clean polished surface of the region can be used. If you choose AUTO, then the

software calculates the reference image from the original image by low pass filtering.

12 Saving and transferring images

12.1 Saving images

Many scientific journals nowadays require that image data included in submitted manuscripts are

also sent in the native format, which contains all acquisition parameters (metadata). Thus, always

save your original images as Zeiss native ZVI format. All metadata is saved within the ZVI file.

Although AxioVision is not able to show all the metadata if you have acquired multidimensional

images, the metadata are still there. These can be found in the meta data xml if the image is

exported. The exposure times of different channels can be shown by annotating the image with

“exposure time”. You can also open the file in ImageJ on the workstations to access the metadata.

Drive H: is your personal local folder. Actually it is a link to the automatically created folder

D:\"your username". You can keep your images here at your own risk (no backups are taken!). It is

recommended to transfer images somewhere else after your imaging session.

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Zeiss Axioplan 2 imaging and Axiophot 2 Guía de usuario Pagina 24