Zeiss Axioplan 2 imaging and Axiophot 2 Guía de usuario descargar pdf (Pagina 19)

Figure 16. Refresh rate settings for live image.

10.3 Acquiring a single image

A single image can be acquired by clicking the snap-button (Figure 17). The new image will open

in a new window.

Figure 17. Snap-button in Axiovision software.

10.4 Exposure time

Click AxioCamHR in the Work area menu (Figure 18) and choose ADJUST tab (Figure 19). There

are three ways of adjusting the time of exposure:

manually, either with a slider or by entering the numeric value

by clicking MEASURE, the software estimates the suitable time of exposure

by choosing AUTOMATIC, the software estimates the time of exposure before each

snapshot

To prevent overexposure, check that the signal intensity histogram does not touch the right end of

the graph (the bright end). Over exposed pixels can be seen as red in the live window by clicking

the over exposure button (looks like a French flag). It will show underexposed pixels in blue.

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Zeiss Axioplan 2 imaging and Axiophot 2 Guía de usuario Pagina 19