Zeiss Axiolab A Especificaciones descargar pdf (Pagina 72)

OPERATION

Carl Zeiss Lighting and contrasting method in transmitted light Axio Lab.A1

72 430037-7144-001 04/2013

4 OPERATION

4.1 Lighting and contrasting method in transmitted light

4.1.1 Adjusting the transmitted light brightfield according to KÖHLER

(1) General principle

Transmitted light brightfield microscopy is the most common optical microscopy method, as high-

contrast or colored specimens (e.g. blood smears) can be viewed quickly and easily.

For a true-to-object imaging, indirect ray bundles, i.e. ray bundles diffracted and scattered on the

specimen details, are of major importance in addition to the so-called direct ray bundles. The higher the

portion of indirect bundles of rays (aperture), the more realistic the microscopic image according to ABBE

will be.

To make use of the microscope's full optical performance, and in particular that of the objective, the

condenser, luminous-field diaphragm and aperture diaphragm should be adjusted in line with the

requirements for KÖHLER illumination. These fundamental rules of microscope adjustment are described

in detail below in Section 4.1.1 (3) "Setting transmitted light brightfield according to KÖHLER".

(2) Instrumentation for transmitted light brightfield

The equipment of every Axio Lab.A1 microscope, except the stand for reflected light, allows the

transmitted light brightfield method to be used.

All available condensers, except special condensers such as darkfield condensers, can be used for the

transmitted light brightfield.

(3) Adjusting the transmitted light brightfield according to KÖHLER

 The Axio Lab.A1 has been started up correctly (see Section3).

 The Axio Lab.A1 is switched on.

x Adjust image brightness with controller for light intensity (Fig. 4-1/2) on microscope stand.

x Insert a high-contrast specimen into the specimen holder of the mechanical stage.

x If condensers with a swiveling front lens are used, swivel these in with t 10x objectives and set the

condenser with drive head for height adjustment (Fig. 4-1/3 or Fig. 4-2/2) to the upper stop. The stop

must be adjusted so that the specimen is not lifted by the condenser (for setting the condenser stop

see Section 4.1.1 (4)).

x When using condensers with a turret/modulator disk and knurled ring (Fig. 4-2/3) set position H

(brightfield).

x Swivel in 10x objective on nosepiece (Fig. 4-1/6) and focus with drive knob (Fig. 4-1/2) on the

specimen.

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Zeiss Axiolab A Especificaciones Pagina 72