Zeiss Axiolab A Especificaciones descargar pdf (Pagina 73)

OPERATION

Axio Lab.A1 Lighting and contrasting method in transmitted light Carl Zeiss

04/2013 430037-7144-001 73

x Close luminous-field diaphragm (Fig. 4-1/5)

until it is visible (even if not in focus) in the field

of view (Fig. 4-1/A).

x Turn the vertical control of the condenser drive

to lower the condenser until the edge of the

luminous-field diaphragm appears in focus

(Fig. 4-1/B).

x Center the luminous-field diaphragm using the

two centering screws (Fig. 4-1/4) on the

condenser carrier (Fig. 4-1/C) and then open the

luminous-field diaphragm until the edge of the

diaphragm just disappears from the field of

vision (Fig. 4-1/D).

x For aperture diaphragm adjustment (contrast)

remove an eyepiece from the tube socket and

look into the socket with the naked eye. Set the

aperture diaphragm with the adjusting lever

(Fig. 4-2/4) to approx. 2/3 ... 4/5 of the

diameter of the exit pupil of the objective

(Fig. 4-1/E). In most applications, this aperture

diaphragm setting provides optimum contrast at

almost ideal resolution, and is therefore the best

compromise for the human eye.

x Reinsert the eyepiece into the tube socket.

Every change of objective will result in a change in specimen field size and objective aperture,

together with a possible slight change in centering, so that for optimum results the luminous-

field and aperture diaphragm adjustments must be repeated.

With objectives < 10x the front lens of the condenser (if swivelable) must be folded out and the

aperture diaphragm completely opened. For better contrast with such large object fields, the

luminous-field aperture can be used by reducing its aperture by a certain amount. Overclosing

should be avoided so as not to impair the uniformity of the illumination of the field of view.

Fig. 4-1 Microscope settings in transmitted

light brightfield

1 2 ... 68 69 70 71 72 73 74 75 76 77 78 ... 117 118

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Zeiss Axiolab A Especificaciones Pagina 73