Zeiss Axiolab A Especificaciones descargar pdf (Pagina 95)

OPERATION

Axio Lab.A1 Lighting and contrasting method in transmitted light Carl Zeiss

04/2013 430037-7144-001 95

x Limit to aperture to a value of about 0.2.

x Turn the rotary stage Pol until the specimen is almost obliterated, i.e. completely dark.

x Rotate the stage once (by 45°) so that the specimen is in a diagonal position (bright).

The interference intensity or color leads to the following conclusion:

 If more or less strong interference colors appear on the specimen, the path difference ranges

approximately between 1/2 O and 5 O.

The suitable compensator is:

tilting compensator B 0-5

 If the specimen-side color changes from light gray/white to a strong interference color, when a

compensator O (473704-0000-000) is inserted in the compensator slot, the path difference is

(1/4 ...1/2) O.

A prerequisite for occurrence of the color change effect may be the evaluation in two specimen

positions rotated at an angle of 90° from one another, plus a centered stage.

The suitable compensator is:

tilting compensator B 0-5

or the compensation method according to DE SENARMONT up to 1 O

using the Senarmont compensator 546/4 nm.

The compensation method according to DE SENARMONT requires the use of the rotatable

analyzer.

 After insertion of the compensator O and rotation of the specimen by 90°, the interference color

remains white; in this case, however, it is a "higher-order white" and thus the path difference is > 5 O.

The suitable compensator is:

tilting compensator K 0-30

(Accessory 000000-1115-698)

 A dark gray as appearing interference color suggests very small path differences (O/10 or 54.6 nm).

The suitable compensator is:

Brace-Köhler rotary compensator

/10 (Accessory 000000-1115-703).

x Insert the compensator into the slit as far as it will go.

The accompanying instructions must be observed for measurement preparation and procedure.

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Zeiss Axiolab A Especificaciones Pagina 95