Zeiss Axiolab A Especificaciones descargar pdf (Pagina 82)

OPERATION

Carl Zeiss Lighting and contrasting method in transmitted light Axio Lab.A1

82 430037-7144-001 04/2013

(3) Setting the microscope

x Set the microscope as for the transmitted light brightfield (see Section 4.1.1), taking care to ensure the

correct interpupillary distance in the binocular tube (see Section 3.5.5).

x Center rotary stage Pol (Fig. 4-5/1) and objectives (see Sections 3.1.8.5 and 3.1.8.6).

x Swivel polarizer (Fig. 4-5/3) into the light path and, if it is rotatable, position it at 0°.

x Insert the analyzer slider (Fig. 4-5/2) into the slit for compensators (if tube does not already have an

analyzer). The field of view will appear dark due to the crossed polarizers. If not, align the analyzer in

the tube or the intermediate plate.

x Set the alignment specimen Pol on the microscope stage and turn to the dark position of the

alignment specimen.

x Remove the analyzer and align the crossline reticle with the cracks in the specimen.

x Subsequently reinsert the analyzer and remove the alignment specimen. The pass directions of the

polarizer and analyzer will now be parallel to the crossline reticle (polarizer EW, analyzer NS).

An adjustment of the crossline reticle

is not necessary when working with the intermediate

plate and the binocular photo tube Pol (425520-9100-000).

x Rotate the rotary stage Pol with the specimen, e.g. a synthetic fiber, until the specimen appears as

dark as possible. In this position, the fiber extends parallel to one of the two directions of the crossline

reticle.

Do not change the interpupillary di

stance on the binocular tube, as the the angle of the

crossline reticle to the fiber will be changed.

x Now turn the stage on by 45° so that the longitudinal axis of the fiber is oriented NE-SW (Fig. 4-8).

The specimen will display the greatest brightness here (diagonal position). In this position the

specimen may have any color.

x Insert the compensator O (only possible if used with screw-in analyzer in tube or intermediate plate).

Like the specimen, the compensator O is a birefringent object, albeit with a defined path difference of

550 nm and the principal direction of oscillation n

definitely oriented in a NE-SW direction.

By moving compensator O into the light path, the specimen changes its color. The type of color change

depends on the orientation of the specimen (NE-SW or NW-SE).

The changes in color are attributable to optical interference. The interference colors (path differences) in

both diagonal positions (NE-SW and NW-SE) of the specimen must be compared in this connection.

The path difference results from the superposition (interference) of the direction of oscillation of the

specimen with the direction of oscillation of the compensator O.

The greater path difference occurs, if the direction of oscillation of the specimen with the absolutely or

relatively highest refractive index (n

or n

J’

) is parallel to the principal direction of oscillation of the

compensator O. The specimen will then appear, for instance, in greenish-blue (Fig. 4-7/2).

The smallest path difference occurs, if the direction of oscillation of the specimen with the absolutely or

relatively lowest refractive index (n

or n

D’

) is perpendicular to the direction of oscillation of the

compensator O. The specimen will then appear, for instance, in yellow (Fig. 4-7/3).

1 2 ... 77 78 79 80 81 82 83 84 85 86 87 ... 117 118

Comentarios a estos manuales

Sin comentarios

Zeiss Axiolab A Especificaciones Pagina 82

Comentarios a estos manuales

Relacionado con productos y manuales para Microscopios Zeiss Axiolab A