Zeiss Axiolab A Especificaciones descargar pdf (Pagina 79)

OPERATION

Axio Lab.A1 Lighting and contrasting method in transmitted light Carl Zeiss

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(3) Setting the microscope

x Set the microscope as in the transmitted light

brightfield according to KÖHLER (see Section

4.1.1 (3)).

x Center rotary stage Pol (Fig. 4-5/1) (see Section

3.1.8.5) and objectives (see Section 3.1.8.6).

x Swivel polarizer (Fig. 4-5/3) into the light path

and, if it is rotatable, position it at 0°.

x Insert the analyzer slider (Fig. 4-5/2) into the slit

for compensators (if tube does not already have

an analyzer). The field of view will appear dark

due to the crossed polarizers. With screw-on

analyzers, care must be taken to ensure that

they are aligned with polarizer D (i.e. crossed

position).

x Move the specimen into the field of view and

turn it with the rotary stage. As a rule, while

being rotated between crossed polarizers,

birefringent (anisotropic) specimens will now

display the variations in color and intensity

described above. However, optically anisotropic

substances may also remain dark, if an isotropic

direction, e.g. of optically uniaxial or biaxial

crystals, is oriented parallel to the direction of

observation.

Fig. 4-5 Components for transmitted light

polarization

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Zeiss Axiolab A Especificaciones Pagina 79