
OPERATION Axioskop 40
Carl Zeiss Illumination and contrast procedures Axioskop 40 FL
3-28 B 40-810 e 12/01
• Replace eyepiece in the tube.
• If the height of the dark field condenser has
been set correctly enough, any visible
brightening in the field of view can be reduced,
and the luminous-field diaphragm image will be
almost perfectly in focus.
• Finally, match the diameter of the luminous-
field diaphragm to the size of the field of view.
☞
Dark field microscopy requires specimens to be considerably cleaner than in other techniques.
Fingerprints, dirt or dust particles, in particular, have negative effects, since they brighten the
background of the field of view and decrease the contrast of the object image.
Fig. 3-23 Centering dark field stop on the
universal condenser
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