Zeiss LSM 510 META Especificaciones Pagina 139
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OPERATION
LSM 510 Image Optimization
B 40-051 e 07/98 4-81
4.7 Image optimization
Single frames
Described below is the example of the acquisition of an image, using an excitation wavelength of 488
nm and a fluorescence emission range above 505 nm.
Let the specimen be a thin section through a stem of Convallaria majalis (Lily-of-the-Valley). The
description applies to the use of the Axioplan microscope, and analogously also to the Axiovert.
Requirements
The suitable laser is switched on.
The specimen has been positioned and focused
for scanning.
The slide rod on the microscope tube has been
pulled out as far as it will go.
Click on the <Config> button in the Acquire
main menu item.
This opens the "Configuration Control"
window.
Click on the <Recording Configuration> button. A
window with the same title will open where you
can select the relevant configuration and activate it
via the <Apply> key.
Open the list box.
Select the necessary configuration (e.g.
488 nm, LP 505).
Activate the selected configuration by clicking
on the <Apply> button.
The Beam Path and Channel Assignment panel
displays the configuration loaded.
☞
The set laser intensity can be subsequently optimized for the current situation via the
<Excitation> button.
Fig. 4-90
- Laser Scanning Microscope 1
- B 40-051 e 07/98 III 3
- IV B 40-051 e 07/98 4
- Scope of Equipment Supplied 6
- INTRODUCTION 10
- X B 40-051 e 07/98 10
- CONTENTS 11
- NOTES ON DEVICE SAFETY 12
- Contents LSM 510 12
- 1-2 B 40-051 e 07/98 12
- 1 NOTES ON DEVICE SAFETY 13
- Exit aperture 18
- B 40-051 e 07/98 1-9 19
- 1-10 B 40-051 e 07/98 20
- LGK 7774 21
- LGK 7628-1 21
- 1-12 B 40-051 e 07/98 22
- LSM 510 - SETUP REQUIREMENTS 24
- 2-2 B 40-051 e 07/98 24
- Fig. 2-1 25
- Fig. 2-2 25
- Fig. 2-3 26
- Fig. 2-4 27
- 2-12 B 40-051 e 07/98 34
- CHAPTER 3 INTRODUCTION TO 35
- LASER SCANNING MICROSCOPY 35
- 3-2 B 40-051 e 07/98 36
- Module VIS 40
- Scan ModuleMicroscope 40
- Module UV 40
- 3-12 B 40-051 e 07/98 46
- CHAPTER 4 OPERATION 59
- 4 OPERATION 61
- Fig. 4-1 62
- Fig. 4-2 63
- Fig. 4-3 63
- Fig. 4-4 64
- Fig. 4-5 65
- Fig. 4-6 66
- Fig. 4-7 66
- Fig. 4-8 67
- Fig. 4-9 67
- Fig. 4-10 68
- Fig. 4-11 69
- Fig. 4-12 70
- Fig. 4-13 71
- Fig. 4-14 72
- Fig. 4-15 73
- Fig. 4-16 74
- Fig. 4-17 75
- Fig. 4-18 76
- Fig. 4-19 77
- Fig. 4-20 78
- Fig. 4-21 79
- Fig. 4-22 80
- Fig. 4-23 82
- Fig. 4-24 82
- Fig. 4-25 83
- Fig. 4-26 83
- Fig. 4-27 83
- Fig. 4-28 84
- Fig. 4-29 84
- Fig. 4-30 85
- Fig. 4-31 86
- Fig. 4-32 88
- Fig. 4-33 89
- Fig. 4-34 90
- Fig. 4-35 90
- Fig. 4-36 91
- Fig. 4-37 92
- Fig. 4-38 93
- Fig. 4-39 95
- Fig. 4-40 96
- Fig. 4-41 97
- Fig. 4-42 98
- Fig. 4-43 100
- Fig. 4-44 101
- Fig. 4-45 103
- OPERATION 104
- 4-46 B 40-51 e 07/98 104
- B 40-051 e 07/98 4-47 105
- Fig. 4-48 106
- Fig. 4-49 107
- Fig. 4-50 108
- Fig. 4-51 109
- Fig. 4-52 110
- Fig. 4-53 111
- Fig. 4-54 112
- Fig. 4-55 113
- Fig. 4-56 114
- Fig. 4-57 115
- Fig. 4-58 115
- Fig. 4-59 116
- Fig. 4-60 117
- Fig. 4-61 118
- Fig. 4-62 119
- Fig. 4-63 120
- Fig. 4-64 121
- Fig. 4-65 122
- Fig. 4-66 123
- B 40-051 e 07/98 4-67 125
- Fig. 4-68 126
- Colored marker positions 126
- Fig. 4-69 127
- Fig. 4-70 128
- Fig. 4-71 129
- Fig. 4-72 129
- Fig. 4-73 130
- Fig. 4-74 130
- Fig. 4-75 131
- Fig. 4-76 131
- Fig. 4-77 132
- Fig. 4-78 133
- Fig. 4-79 133
- Fig. 4-81 134
- Fig. 4-80 134
- Fig. 4-82 135
- Fig. 4-83 135
- Fig. 4-90 139
- Fig. 4-91 140
- LSM 510 Image Optimization 141
- B 40-051 e 07/98 4-83 141
- Fig. 4-93 142
- Fig. 4-94 143
- Fig. 4-95 144
- B 40-051 e 07/98 4-87 145
- Fig. 4-97 146
- Fig. 4-98 147
- Fig. 4-99 148
- Fig. 4-100 149
- Fig. 4-101 150
- Fig. 4-102 151
- Fig. 4-103 152
- Fig. 4-104 153
- / Ampl. Gain 154
- Fig. 4-105 155
- Fig. 4-106 156
- Fig. 4-107 157
- Fig. 4-108 158
- Fig. 4-109 159
- Fig. 4-110 160
- Fig. 4-111 161
- Fig. 4-112 162
- Fig. 4-113 163
- Fig. 4-114 164
- Fig. 4-115 164
- Fig. 4-116 165
- Fig. 4-117 166
- Fig. 4-118 168
- Fig. 4-119 169
- Fig. 4-120 170
- Fig. 4-121 171
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- Fig. 4-123 173
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- Fig. 4-125 175
- Fig. 4-126 176
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- Fig. 4-128 178
- Fig. 4-129 179
- Fig. 4-130 180
- Fig. 4-131 181
- 4-124 B 40-051 e 07/98 182
- Fig. 4-133 183
- Fig. 4-134 185
- Fig. 4-135 186
- Fig. 4-136 187
- Fig. 4-137 188
- Fig. 4-138 188
- Fig. 4-139 189
- Fig. 4-140 190
- Fig. 4-141 191
- 4-134 B 40-051 e 07/98 192
- Fig. 4-143 193
- Fig. 4-144 194
- B 40-051 e 07/98 4-137 195
- Fig. 4-146 196
- Fig. 4-147 196
- Fig. 4-148 198
- Fig. 4-149 199
- Fig. 4-150 199
- Fig. 4-151 199
- Fig. 4-152 200
- Fig. 4-153 201
- Fig. 4-154 202
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- Fig. 4-160 206
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- Fig. 4-164 210
- Fig. 4-165 212
- Fig. 4-166 214
- Fig. 4-167 215
- Fig. 4-168 216
- Fig. 4-169 217
- LSM 510 Annex 219
- B 40-051 e 07/98 4-161 219
- Annex LSM 510 220
- 4-162 B 40-051 e 07/98 220
- B 40-051 e 07/98 4-163 221
- 4-164 B 40-051 e 07/98 222
- B 40-051 e 07/98 4-165 223
- 4-166 B 40-051 e 07/98 224
- B 40-051 e 07/98 4-167 225
- 4-168 B 40-051 e 07/98 226
- B 40-051 e 07/98 4-169 227
- 4-170 B 40-051 e 07/98 228
- Fig. 4-170 Filter change 229
- Allen key 230
- B 40-051 e 07/98 4-173 231
- Fine focusing 233
- Fig. 4-173 234
- Fig. 4-174 237
- Fig. 4-175 240
- Fig. 4-176 246
- Fig. 4-177 247
- Fig. 4-178 249
- Fig. 4-179 250
- Fig. 4-180 252
- Fig. 4-181 253
- CHAPTER 5 3D FOR LSM 510 255
- 5-2 B 40-051 e 07/98 256
- Positive rotation directions 257
- Fig. 5-1 257
- Fig. 5-2 259
- Display window 260
- Fig. 5-4 265
- Fig. 5-5 267
- Fig. 5-6 268
- Fig. 5-7 270
- Fig. 5-9 274
- Fig. 5-10 275
- Fig. 5-11 276
- Fig. 5-12 278
- Fig. 5-13 280
- Fig. 5-14 283
- Fig. 5-15 285
- Fig. 5-16 287
- Fig. 5-17 288
- Fig. 5-18 291
- Fig. 5-19 291
- Fig. 5-20 292
- Fig. 5-21 293
- Fig. 5-22 295
- Fig. 5-23 297
- Fig. 5-24 299
- Fig. 5-25 301
- Fig. 5-26 303
- Fig. 5-27 304
- Fig. 5-28 305
- Fig. 5-29 306
- Fig. 5-30 307
- Fig. 5-31 308
- Fig. 5-32 309
- Fig. 5-33 310
- Fig. 5-34 314
- Functions LSM 510 318
- 5-64 B 40-051 e 07/98 318
- Measurement Concept 318
- Fig. 5-36 319
- Fig. 5-37 320
- Fig. 5-39 327
- Measured 329
- Object Features 329
- Volume Features 329
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